Eli binzana libuza umbuzo: "Ke, luhlobo luni lwe-vial ye-cryogenic le ukuba ayinakusetyenziswa kwi-nitrogen engamanzi?"
Akukho veki idlulayo apho singacelwa ukuba sichaze le nto ibonakala ingaqhelekanga ivela kwiphepha ngalinye lemveliso ye-cryovial nokuba ngubani na umenzi, nokuba ungakanani umthamo kwaye nokuba ngumsonto wangaphakathi we-cryovial okanye intambo yangaphandle ye-cryovial.
Impendulo yile: Lo ngumcimbi woxanduva kwaye ayingombuzo malunga nomgangatho we-cryovial.
Makhe sicacise.
Njengeetyhubhu ezininzi ezihlala ixesha elide, ii-cryovials zenziwe nge-polypropylene ezinzileyo yobushushu.
Ubunzima bepolypropylene bumisela uluhlu olukhuselekileyo lokushisa.
Uninzi lwe-15mL kunye ne-50mL yeityhubhu ezibumbene zineendonga ezibhityileyo ezinciphisa ukusebenza kwazo kubushushu obungekho ngaphantsi kwe -86 ukuya -90 ngokukaCelsius.
Iindonga ezibhityileyo zikwachaza ukuba kutheni i-15mL kunye ne-50mL iityhubhu ezikhonkotha zingacetyiswa ukuba zijikelezwe ngamazinga akhawulezayo kune-15,000xg njengoko iplastiki ithanda ukuqhekeka kwaye iqhekeke ukuba iqhutywa ngaphaya kwalo mda.
Iingqayi ze-Cryogenic zenziwe nge-polypropylene engqindilili ezivumela ukuba zibambe phantsi kwamaqondo obushushu abandayo kwaye zijikelezwe kwi-centrifuge ngesantya esingaphezulu kwe-25,000xg okanye ngaphezulu.
Ingxaki ilele kumnqwazi wokutywina osetyenziselwa ukukhusela i-cryovial.
Ukuze i-cryovial ikhusele ngokufanelekileyo izicubu, iseli okanye isampuli yentsholongwane equlethwe kuyo, i-cap kufuneka ijije ngokupheleleyo kwaye yenze itywina elingavuziyo.
Esona sikhewu sincinci siyakuvumela ukuvela kwamanzi kunye nokungcoliseka komngcipheko.
Iinzame ezibukhali zenziwa ngabavelisi be-cryovial ukuvelisa itywina elikumgangatho ophezulu elinokubandakanya isilicon o-ring kunye/okanye umsonto oshinyeneyo wokukrola ikepusi phantsi ngokupheleleyo.
Lo ngumlinganiselo wento eyenziwa ngumenzi we-cryovial.
Ekugqibeleni impumelelo okanye ukusilela kwe-cryovial ukugcina isampuli yokuwa kwi-lab technician ukuqinisekisa ukuba isitywina esilungileyo senziwe.
Ukuba itywina alilunganga, kwaye nakwiimeko apho i-cap ivalwe ngokufanelekileyo, initrogen engamanzi inokungena kwi-cryovial xa intywiliselwa kwi-nitrogen engamanzi engamanzi.
Ukuba isampuli inyibilika ngokukhawuleza kakhulu, initrogen engamanzi iya kwanda ngokukhawuleza kwaye ibangele iziqulatho ezicinezelekileyo ziqhume kwaye zithumele iingceba zeplastiki ezandleni nasebusweni bakhe nabani na onelishwa lokuba abe kufutshane.
Ke ngoko, ngaphandle kokungaqhelekanga, abavelisi be-cryovial bafuna abasasazi babo ukuba babonise ngesibindi isilawuli sokungasebenzisi i-cryovials yabo ngaphandle kwesigaba segesi senitrogen engamanzi (emalunga ne-180 ukuya kwi-186C).
Usenako ukukhawuleza udanyaze imixholo yokukhenkceza kwi-cryovial ngokuyintywilisela ngokuyinxenye kwisigaba solwelo senitrogen;zomelele ngokwaneleyo kwaye aziyi kuqhekeka.
Ngaba uyafuna ukufunda ngakumbi malunga neengozi zokugcina iingqayi ze-cryogenic kwisigaba solwelo senitrogen?
Nali inqaku elivela kwiZiko le-UCLA loKhuseleko lweLabhoratri ebhala ukwenzakala ngenxa yogqabhuko-dubulo lwe-cryovial.
Ixesha lokuposa: Apr-21-2022